Composite

Part:BBa_K2868021:Design

Designed by: Cale Lester   Group: iGEM18_Stanford-Brown-RISD   (2018-10-09)


mfp151 with 6x his-tag expression system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 860
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When ordering this construct to be synthesized using IDT gBlocks fragments the repeats made it unable to be ordered all at once. It had to be split into two orders and both fragments then had to be gibson assembled together into the plasmid at the same time in order to be used.


Source

mfp151 is a recombinant protein based off mfp1 and mfp5 which are adhesive proteins naturally produced in mussels. T7 promoter and terminator are based off parts in the T7 lambda bacteriophage.

References